Publications

Multiplexed single-molecule analysis of barcoded protein variants

Stefan H. Mueller, David M. Grimson, Holden Paz, Aidan C. Fitch, Haibo Yu, Marco Ribezzi-Crivellari, Andrew D. Griffiths, Antoine M. Van Oijen, Lisanne M. Spenkelink

Nature Communications · 2026

Structure of the pre-initiation complex explains CMGE biogenesis

Thomas Pühringer, Berta Canal, Giacomo Palm, Agata Butryn, Emma C. Couves, Oliver Willhoft, Jacob S. Lewis, John F. X. Diffley, Alessandro Costa

Nature · 2026

Direct visualisation of post-replication gap formation at the bacterial RRS

Nicholas Kusi-Appauh, Phuong Pham, Elise M. Wilkinson, Michael M. Cox, Jacob S. Lewis, Myron F. Goodman, Lisanne M. Spenklink

bioRxiv · 2026

ATP is dispensable for E. coli DNA replication and eukaryotic helicase activity

Richard R. Spinks, Aleksa Lakic, Celine Kelso, Slobodan Jergic, Olga Yurieva, Zhi-Qiang Xu, Michael E. O'Donnell, Nicholas E. Dixon, Antoine M. Van Oijen, Jacob S. Lewis, Lisanne M. Spenkelink

Nature Communications · 2026

The interplay between supercoiling and DNA modifying enzymes at the single-molecule level

Elise M. Wilkinson, Antoine M. Van Oijen, Timothy D. Craggs, Stefan H. Mueller, Lisanne M. Spenkelink

Scientific Reports · 2026

Mechanistic Insights into Nanobody-Based Quenchbody Sensing via Structural Modeling and Molecular Simulations

Nehad S. El Salamouni, Jordan H. Cater, Qiang Zhu, Lisanne M. Spenkelink, Haibo Yu

Journal of Chemical Information and Modeling · 2026

nCas9-based method for rolling-circle DNA substrate generation

Nischal Sharma, Kelsey S. Whinn, Harshad Ghodke, Antoine M. Van Oijen, Jacob S. Lewis, Lisanne M. Spenkelink

Analytical Biochemistry · 2025

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Nicholas Kusi-Appauh, Stefan H. Mueller, Stephen F. Ralph, Olga Yurieva, Michael E. O'Donnell, Jacob S. Lewis, Lisanne M. Spenkelink

Journal of Visualized Experiments · 2025

Nanobody engineering: computational modelling and design for biomedical and therapeutic applications

Nehad S. El Salamouni, Jordan H. Cater, Lisanne M. Spenkelink, Haibo Yu

FEBS Open Bio · 2024

Insight into Single-Molecule Imaging Techniques for the Study of Prokaryotic Genome Maintenance

Nischal Sharma, Antoine M. Van Oijen, Lisanne M. Spenkelink, Stefan H. Mueller

Chemical & Biomedical Imaging · 2024

Embracing Heterogeneity: Challenging the Paradigm of Replisomes as Deterministic Machines

Jacob Lewis, Antoine Van Oijen, Lisanne M. Spenkelink

Chemical Reviews · 2023

Understanding G-Quadruplex Biology and Stability Using Single-Molecule Techniques

Nicholas Kusi-Appauh, Stephen F. Ralph, Antoine M. Van Oijen, Lisanne M. Spenkelink

Journal of Physical Chemistry B · 2023

Observing protein dynamics during DNA-lesion bypass by the replisome

Elise M. Wilkinson, Lisanne M. Spenkelink, Antoine M. Van Oijen

Frontiers in Molecular Biosciences · 2022

Single-Molecule Insights Into the Dynamics of Replicative Helicases

Richard R. Spinks, Lisanne M. Spenkelink, Nicholas E. Dixon, Antoine M. Van Oijen

Front. Mol. Biosci. · 2021

DnaB helicase dynamics in bacterial DNA replication resolved by single-molecule studies

Richard R. Spinks, Lisanne M. Spenkelink, Sarah A. Stratmann, Zhi-Qiang Xu, N. Patrick J. Stamford, Susan E. Brown, Nicholas E. Dixon, Slobodan Jergic, Antoine M. Van Oijen

Nucleic Acids Research · 2021

Biology on track: single-molecule visualisation of protein dynamics on linear DNA substrates

Gurleen Kaur, Lisanne M. Spenkelink

Essays in Biochemistry · 2021