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Multiplexed single-molecule analysis of barcoded protein variants
Single-molecule techniques provide exceptional resolution of biomolecular dynamics but are limited by low throughput. Here, we present …
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Multiplexed single-molecule analysis of barcoded protein variants
Structure of the pre-initiation complex explains CMGE biogenesis
When cells enter S phase, bidirectional DNA replication is initiated through the kinase-regulated recruitment of three activators …
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Structure of the pre-initiation complex explains CMGE biogenesis
ATP is dispensable for E. coli DNA replication and eukaryotic helicase activity
Adenosine triphosphate (ATP) hydrolysis is the main cellular source of energy used to drive biochemical reactions that are otherwise …
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ATP is dispensable for E. coli DNA replication and eukaryotic helicase activity
The interplay between supercoiling and DNA modifying enzymes at the single-molecule level
DNA supercoiling refers to the overwinding or underwinding of the double helix, forming plait-like structures called plectonemes. …
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The interplay between supercoiling and DNA modifying enzymes at the single-molecule level
Mechanistic Insights into Nanobody-Based Quenchbody Sensing via Structural Modeling and Molecular Simulations
Nanobody-based quenchbodies are powerful fluorescent sensors for detecting biomolecular targets. They rely on tryptophan residues in …
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Mechanistic Insights into Nanobody-Based Quenchbody Sensing via Structural Modeling and Molecular Simulations
nCas9-based method for rolling-circle DNA substrate generation
Rolling-circle DNA replication is a DNA-duplication mechanism whereby circular DNA templates are continuously copied to produce long …
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nCas9-based method for rolling-circle DNA substrate generation
Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
The ability of proteins involved in eukaryotic DNA replication to overcome obstacles — such as protein and DNA ‘roadblocks’ …
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Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
Nanobody engineering: computational modelling and design for biomedical and therapeutic applications
Nanobodies, the smallest functional antibody fragment derived from camelid heavy-chain-only antibodies, have emerged as powerful tools …
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Nanobody engineering: computational modelling and design for biomedical and therapeutic applications
Insight into Single-Molecule Imaging Techniques for the Study of Prokaryotic Genome Maintenance
Genome maintenance comprises a group of complex and interrelated processes crucial for preserving and safeguarding genetic information …
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Insight into Single-Molecule Imaging Techniques for the Study of Prokaryotic Genome Maintenance
Embracing Heterogeneity: Challenging the Paradigm of Replisomes as Deterministic Machines
The paradigm of cellular systems as deterministic machines has long guided our understanding of biology. Advancements in technology and …
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Embracing Heterogeneity: Challenging the Paradigm of Replisomes as Deterministic Machines